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JoVE Journal
Bioengineering

This content is Open Access.

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
 

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Article doi: 10.3791/61938
May 17th, 2021

Summary May 17th, 2021

This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and hypoxia.

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