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Bioengineering
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异条件下具有不同刚度的聚乙烯醇-钴-衣康酸水凝胶的人多能干细胞培养
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
Please note that all translations are automatically generated.
Click here for the English version.
异条件下具有不同刚度的聚乙烯醇-钴-衣康酸水凝胶的人多能干细胞培养
DOI:
10.3791/57314-v
•
11:37 min
•
February 03, 2018
•
Tzu-Cheng Sung*
1
,
Hsing-Fen Li*
1
,
Akon Higuchi
2
,
Qing-Dong Ling
4
,
Jia-Sin Yang
,
Yeh-Chia Tseng
,
Chih-Hsien Pan Pan
,
Abdullah A. Alarfaj
,
Murugan A. Munusamy
,
Suresh Kumar
,
Shih-Tien Hsu
,
Kadarkarai Murugan
8
1
Department of Chemical and Materials Engineering
,
National Central University
,
2
Department of Botany and Microbiology
,
King Saud University
,
3
Cathay Medical Research Institute
,
Cathay General Hospital
,
4
Graduate Institute of Systems Biology and Bioinformatics
,
National Central University
,
5
Department of Medical Microbiology and Parasitology
,
Universiti Putra Malaysia
,
6
Department of Internal Medicine
,
Taiwan Landseed Hospital
,
7
Department of Zoology
,
Bharathiar University
,
8
Thiruvalluvar University
Chapters
00:04
Title
01:00
Polyvinyl Alcohol-Co-Itaconic Acid (P-IA) Hydrogel Dish Preparation and Grafting
05:02
Passaging of Undifferentiated Human Embryonic Stem (ES) and Human Induced Pluripotent Stem (iPS) Cells on Hydrogels
06:37
Characterization of the Expression of Pluripotency Proteins by Immunostaining
08:49
Results: Human ES and iPS Pluripotency on Conventional Dishes and Hydrogel Dishes
10:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
提出了一种不同刚度的聚乙烯醇-共衣酸凝胶的制备方法, 并与无肽接枝, 研究了生物材料的硬度对其分化和增殖的影响。干细胞。水凝胶的刚度受交联时间的控制。
Tags
Polyvinyl Alcohol-co-itaconic Acid Hydrogels
Stem Cell Culture
Xeno-free Conditions
Hydrogel Elasticity
Stem Cell Differentiation
Crosslinking
Hydrogel Preparation
P-IA Films
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