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Polyvinyl शराब पर मानव Pluripotent स्टेम सेल संस्कृति-सह-Itaconic एसिड Hydrogels के तहत अलग कठोरता के साथ एक्सो-मुक्त शर्तों
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1Department of Chemical and Materials Engineering,National Central University, 2Department of Botany and Microbiology,King Saud University, 3Cathay Medical Research Institute,Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics,National Central University, 5Department of Medical Microbiology and Parasitology,Universiti Putra Malaysia, 6Department of Internal Medicine,Taiwan Landseed Hospital, 7Department of Zoology,Bharathiar University, 8Thiruvalluvar University
Chapters
- 00:04Title
- 01:00Polyvinyl Alcohol-Co-Itaconic Acid (P-IA) Hydrogel Dish Preparation and Grafting
- 05:02Passaging of Undifferentiated Human Embryonic Stem (ES) and Human Induced Pluripotent Stem (iPS) Cells on Hydrogels
- 06:37Characterization of the Expression of Pluripotency Proteins by Immunostaining
- 08:49Results: Human ES and iPS Pluripotency on Conventional Dishes and Hydrogel Dishes
- 10:19Conclusion
एक प्रोटोकॉल polyvinyl शराब तैयार करने के लिए प्रस्तुत किया जाता है-सह-itaconic एसिड hydrogels बदलती कठोरता के साथ, जो और बिना oligopeptides के साथ भ्रष्टाचार कर रहे थे, के भेदभाव और प्रसार पर की कठोरता के प्रभाव की जांच स्टेम सेल । crosslinking समय hydrogels की कठोरता को नियंत्रित किया गया था ।
