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Immunology and Infection

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JoVE Journal Immunology and Infection
L’Assemblée et la Purification du Prototype spumavirus Intasomes
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L’Assemblée et la Purification du Prototype spumavirus Intasomes

Article DOI: 10.3791/57453-v • 10:20 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Recombinant intégrase rétrovirale et oligomères d’ADN imitant les extrémités de l’ADN virales peuvent former un complexe enzymatiquement actif connu comme un intasome. Intasomes peut être utilisé pour les études cinétiques, structurales et biochimiques. Ce protocole détaille comment assembler et purifier le prototype spumavirus intasomes.

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Immunologie et Infection numéro 133 rétrovirus Prototype spumavirus Intasome l’intégrase assemblage complexe de protéine Purification des protéines complexes
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