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Journal / Biology / Isolering og karakterisering høy gjennomstrømning …
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Isolering og karakterisering høy gjennomstrømning ekstracellulære Flux analyse av musen primære nyre rørformede epitelceller
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Isolering og karakterisering høy gjennomstrømning ekstracellulære Flux analyse av musen primære nyre rørformede epitelceller

Article DOI: 10.3791/57718-v • 09:40 min • June 20th, 2018
June 20th, 2018 •
1,2, 1,2, 2,3,4,5
1Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine

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Denne protokollen gir en kostnadseffektiv tilnærming for å isolere og karakterisere musen primære nyre rørformede celler som kan senere sub kultivert for å vurdere nyre biologiske funksjoner ex vivo, inkludert mitokondrie bioenergi.

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Biologi problemet 136 primær cellen aisolering primære celle karakterisering rørformede epitelceller nyre bioenergi mitokondrier åndedrett høy gjennomstrømning ekstracellulære flux analyse fettsyrer oksidasjon kronisk nyresykdom
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