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Bioengineering

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JoVE Journal Bioengineering
En hurtig Image-baserede bakteriel virulens analyse ved hjælp af Amoeba
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En hurtig Image-baserede bakteriel virulens analyse ved hjælp af Amoeba

Article DOI: 10.3791/57844-v • 05:22 min • June 27th, 2018
June 27th, 2018 •
1, 1,2
1Department of Physics and Astronomy, University of California, 2Department of Molecular Biology and Biochemistry, University of California

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Vi præsenterer her, en protokol for at måle virulens af planktoniske eller overflade-attached bakterier ved hjælp af D. discoideum (amøbe) som vært. Virulens måles over en periode på 1 time og vært dræbe kvantificeres ved hjælp af Fluorescens mikroskopi og billede analyse. Vi demonstrere denne protokol ved hjælp af bakterien P. aeruginosa.

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Bioteknologi sag 136 Pseudomonas aeruginosa knyttet til overfladen Planktontaxas Dictyostelium discoideum hurtig virulens assay virulens i biofilm
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