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Journal / Developmental Biology / Analisi trascrittomica unicellulare delle cellule …
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JoVE Journal Developmental Biology
Analisi trascrittomica unicellulare delle cellule Endocrine del pancreas del Mouse
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Analisi trascrittomica unicellulare delle cellule Endocrine del pancreas del Mouse

Article DOI: 10.3791/58000-v • 10:05 min • September 30th, 2018
September 30th, 2018 •
*1,2, *1,2, *1, *1,3, *1,3, 1
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 2Academy for Advanced Interdisciplinary Studies, Peking University, 3PKU-Tsinghua-NIBS Graduate Program, Peking University
* These authors contributed equally

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Descriviamo un metodo per l'isolamento di cellule endocrine da pancreas embrionale, neonatale e postnatale, seguite dal sequenziamento di RNA di cella singola. Questo metodo consente analisi dello sviluppo del pancreas endocrino lignaggio, eterogeneità e trascrittomica dinamiche di cella.

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Biologia inerente allo sviluppo problema 139 Pancreas endocrino delle cellule delle cellule β unicellulare RNA-sequenziamento espressione genica eterogeneità cellulare
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