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Journal / Developmental Biology / Hydrogenion-mediert siRNA Gene-stanse i voksen…
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Developmental Biology

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JoVE Journal Developmental Biology
Hydrogenion-mediert siRNA Gene-stanse i voksen sebrafisk hjerte
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Hydrogenion-mediert siRNA Gene-stanse i voksen sebrafisk hjerte

Article DOI: 10.3791/58054-v • 09:12 min • July 29th, 2018
July 29th, 2018 •
1, 2, 1, 1, 2, 1
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

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Det er fortsatt en stor utfordring å utvikle betinget gen-knockout eller effektiv gen-knockdown i voksen sebrafisk organer. Her kan vi rapportere en protokoll for utøvende hydrogenion-mediert siRNA gen-stanse i voksen sebrafisk hjerte, noe som gir en ny tap-av-funksjonen metoden for å studere voksen organer i sebrafisk og andre modellen organismer.

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Utviklingspsykologi biologi problemet 137 sebrafisk voksen hjertet gjenfødelse nanopartikler siRNA gene stanse utviklingspsykologi biologi genetikk
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