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小鼠幼犬慢性睡眠剥夺的温和处理方法
Chapters
Summary October 11th, 2018
Please note that all translations are automatically generated.
Click here for the English version.
我们描述一种被称为温和处理的睡眠剥夺技术, 调查人员在任何时候都轻轻地对老鼠进行睡眠行为观察。这种方法是一个强大的工具, 让研究人员研究慢性睡眠限制在整个发展可能对未来的大脑生理学和行为的影响。
Transcript
这种方法可以帮助回答睡眠领域的关键问题，如产后发育过程中慢性睡眠限制的影响。这种技术的主要优点是，它的压力比它的其他睡眠剥夺技术小，可以很容易地在整个发展过程中使用，并持续到成年。首先将每个笼子分配给睡眠剥夺或对照组。
确保小鼠能够获得食物和水，并移除任何可能限制小鼠视野的食物、床上用品或富集物。对于对照组，在产后第五天从育种者笼中取下陛下，然后与幼崽一起离开大坝，直到打奶。接下来，将分配给对照组的小鼠移动到睡眠剥夺室，并在实验期间每天用画笔连续用画笔轻轻推动小鼠10分钟。
10分钟后，将老鼠返回动物室，不要打扰它们。首先在产后第五天将陛下从饲养员笼中移走，然后与幼崽一起离开大坝，直到打奶。在光周期内，每天将分配给睡眠剥夺组的小鼠移动到睡眠剥夺室，在那里进行监测。
在此期间，如果怀疑鼠标睡着了，则用画笔轻轻抚摸鼠标，直到观察到反应。或者，反转小鼠，推上它们的背部，扰乱睡眠。如果出现以下任何情况，则考虑让鼠标处于睡眠状态：抽搐、不活动或眼睛闭着。
如果老鼠四处走动，试图在背上翻过来，或者打扮自己，那么它们就清醒了。如果大坝在睡眠不足期间覆盖幼崽，则轻轻地将她从幼崽中推离，这样幼崽的视野是不受限制的。继续使用睡眠剥夺协议，直到实验终结点。
当三个小时的睡眠剥夺期完成时，将床上用品和任何可能被取出的食物放回笼子，然后盖回笼子上。最后，将笼子回到动物的持有室，直到第二天睡眠不足。经过一个月的恢复期后，结果显示，在社交能力测试中，睡眠受限女性比控制雌性多时间嗅探陌生人的老鼠，睡眠受限的男性与对照男性相比，有花更多的时间嗅探物体。
此外，在社会新奇测试阶段，睡眠受限的雌性小鼠比雌性对等对等小鼠花费更多的时间嗅探新小鼠。在尝试此程序时，重要的是要记住注意并及时响应睡眠前的行为，尤其是在睡眠需求很高的新生儿小鼠中。按照这个程序，其他方法，如开放领域，旋转，高架加迷宫，和社会行为测试可以执行，以回答其他问题，如慢性睡眠限制在发育过程中的长期行为影响。
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