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Journal / Immunology and Infection / PCR quantitativo do bacteriófago T7 de Biopanning
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Immunology and Infection

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PCR quantitativo do bacteriófago T7 de Biopanning
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PCR quantitativo do bacteriófago T7 de Biopanning

Article DOI: 10.3791/58165-v • 05:42 min • September 27th, 2018
September 27th, 2018 •
1, 1, 1, 1, 1
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin

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Um tempo, preciso e reprodutível quantitativo PCR (qPCR) método eficiente para enumerar do bacteriófago T7 é descrito aqui. O protocolo descreve claramente a preparação de DNA genômica do fago, preparação de reação de PCR, qPCR ciclismo condições e análise de dados de qPCR.

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Imunologia e infecção edição 139 fago T7 fagos DNA genômico qPCR ensaio de placa limite de detecção escala de quantificação
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