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JoVE Journal
Genetics

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可抽税抗生素耐药盒 P1 转导制备大肠杆菌中的基因缺失
 
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可抽税抗生素耐药盒 P1 转导制备大肠杆菌中的基因缺失

Article DOI: 10.3791/58267-v 08:13 min September 1st, 2018
September 1st, 2018

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Summary

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Click here for the English version.

在这里, 我们提出了使用预先存在的抗生素耐药性-盒式删除结构作为一个基础, 使删除突变体在其他大肠杆菌菌株。这种删除突变可以调动和插入到相应的受体菌株的位置使用 P1 噬菌体转导。

Tags

遗传学 问题 139 抗生素耐药性盒 删除诱变 FLP recombinase P1 转导 易位和组装模块 三联 autotransporter 黏附
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