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Biochemistry

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JoVE Journal Biochemistry
Isolamento de glomérulos e In Vivo de rotulagem das proteínas de superfície celular Glomerular
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Isolamento de glomérulos e In Vivo de rotulagem das proteínas de superfície celular Glomerular

Article DOI: 10.3791/58542-v • 09:12 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 2, 1, 1, 1, 1, 1, 1
1Department of Nephrology, Medical Faculty, Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

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Aqui nós apresentamos um protocolo para murino na vivo rotulagem das proteínas de superfície celular glomerular com biotina. Este protocolo contém informações sobre como perfundir os rins do rato, isolar glomérulos e executar endógena imunoprecipitação da proteína de interesse.

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Bioquímica edição 143 In vivo biotinylation rotulagem de proteína de superfície celular glomérulos isolamento de glomérulos grânulos magnéticos endocitose na vivo tráfico de proteína
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