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Environment
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木本植物的赛莱默水分布,用低温扫描电子显微镜可视化
JoVE Journal
Environment
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JoVE Journal
Environment
Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope
Please note that all translations are automatically generated.
Click here for the English version.
木本植物的赛莱默水分布,用低温扫描电子显微镜可视化
DOI:
10.3791/59154-v
•
10:47 min
•
June 20, 2019
•
Kenichi Yazaki
,
Mayumi Y. Ogasa
,
Katsushi Kuroda
,
Yasuhiro Utsumi
,
Peter Kitin
,
Yuzou Sano
1
Department of Plant Ecology
,
Forestry and Forest Products Research Institute (FFPRI)
,
2
Kansai Research Center
,
Forestry and Forest Products Research Institute (FFPRI)
,
3
Department of Wood Properties and Processing
,
Forestry and Forest Products Research Institute (FFPRI)
,
4
Faculty of Agriculture
,
Kyushu University
,
5
Department of Bacteriology
,
University of Wisconsin
,
6
Research Faculty of Agriculture
,
Hokkaido University
Chapters
00:04
Title
01:14
Sampling: Tension Relaxation within Water Column of Xylem Conduits
03:00
Freeze Fixation with Liquid Nitrogen
04:04
Specimen Preparation
05:49
Transfer to the Cryo-SEM Specimen Chamber
06:30
Freeze-etching
07:00
Results: Xylem Water Distribution in Woody Plants
09:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
观察木本植物中的水分布,可提供有关木本植物水流动力学的重要信息。在这项研究中,我们演示了使用低温和低温-SEM来观察原位赛莱默水分布的实用方法,消除了样品制备过程中水状态的伪性变化。
Tags
Xylem
Water Distribution
Cryo-scanning Electron Microscope
Freeze-fixation
Xylem Tension
Water Potential
Cavitation
Xylem Cells
Aerotome
Woody Plants
Water Regime
Seasonal Variation
Biotic Stress
Freeze/thaw Cycle
Wet Wood
Sampling Procedure
Pressure Chamber
Relaxation Treatment
Water Surface
Transpiration
Vessel Length
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