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Journal / Genetics / Overexpressing RNAs Noncoding זמן באמצעות הפעלת…
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Genetics

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JoVE Journal Genetics
Overexpressing RNAs Noncoding זמן באמצעות הפעלת גנים CRISPR
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Overexpressing RNAs Noncoding זמן באמצעות הפעלת גנים CRISPR

Article DOI: 10.3791/59233-v • 13:04 min • March 1st, 2019
March 1st, 2019 •
1, 1, 2, 1, 3, 3, 1, 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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טכניקות ביטוי מסורתיים מבוססי cDNA יש של תחולה מוגבלת עבור ביטוי של RNAs noncoding זמן עקב שלהם טפסים splice מרובים עם פונקציונליות פוטנציאליים. ביקורת זו דוחות באמצעות טכנולוגיית CRISPR את overexpress וריאציות splice מרובים של RNA זמן noncoding פרוטוקול.

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