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JoVE Journal Bioengineering
Måling af spin-Lattice afslapning magnetisk felt afhængighed af Hyperpolariseret [1-13C] pyruvat
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Måling af spin-Lattice afslapning magnetisk felt afhængighed af Hyperpolariseret [1-13C] pyruvat

Article DOI: 10.3791/59399-v • 11:57 min • September 13th, 2019
September 13th, 2019 •
1, 2, 1,2,3
1Department of Medical Biophysics, Western University, 2Imaging Research Laboratory, Robarts Research Institute, Western University, 3Ontario Institute for Cancer Research

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Vi præsenterer en protokol til at måle den magnetiske felt afhængighed af spin-gitter afslapningstid på 13C-beriget forbindelser, hyperpolariseret ved hjælp af dynamisk nuklear polarisering, ved hjælp af hurtig felt-cyklet relaxometri. Konkret har vi demonstreret dette med [1-13C] pyruvat, men protokollen kunne udvides til andre hyperpolariserede substrater.

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Bioengineering pyruvat spin-Lattice afslapning dynamisk nuklear polarisering felt-cykling relaxometri nuklear magnetisk afslapning dispersion (NMRD) hyperpoloarization
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