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JoVE Journal Genetics
Evaluering af en universel indlejret reverse transkription polymerase kædereaktion til påvisning af Lyssavira
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Evaluering af en universel indlejret reverse transkription polymerase kædereaktion til påvisning af Lyssavira

Article DOI: 10.3791/59428-v • 08:10 min • May 2nd, 2019
May 2nd, 2019 •
*1, *1,2, 1, 1, 1, 1, 1,3, 1
1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
* These authors contributed equally

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En pan-lyssavirus indlejret reverse transkription polymerase kædereaktion er blevet udviklet til at påvise specifikt alle kendte Lyssavira. Validering ved hjælp af rabies hjernen prøver af forskellige dyrearter viste, at denne metode har en følsomhed og specificitet svarende til den gyldne standard fluorescerende antistof test og kunne anvendes til rutinemæssig rabies diagnose.

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Genetik molekylær biologi X rabies lyssavirus indlejret RT-PCR diagnose
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