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Geração rápida de culturas de melanócitos e fibroblastos de Murina primária
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Geração rápida de culturas de melanócitos e fibroblastos de Murina primária

Article DOI: 10.3791/59468-v • 06:32 min • June 26th, 2019
June 26th, 2019 •
1, 1, 1,2
1Department of Cancer Biology and Genetics, The Ohio State University, 2Department of Molecular Genetics, The Ohio State University

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Este protocolo esboça um método rápido para gerar simultaneamente culturas do melanócito e do fibroblasto da pele de 0-4 ratos velhos do dia. Estas culturas primárias podem ser mantidas e manipuladas in vitro para estudar uma variedade de processos fisiologicamente relevantes, incluindo biologia celular da pele, pigmentação, cicatrização de feridas e melanoma.

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Pesquisa do cancro edição 148 melanocyte rato pele isolação cultura de pilha
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