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Immunology and Infection

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JoVE Journal Immunology and Infection
Test ELISA in vitro per valutare la potenza del vaccino contro la rabbia
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Test ELISA in vitro per valutare la potenza del vaccino contro la rabbia

Article DOI: 10.3791/59641-v • 09:04 min • May 11th, 2020
May 11th, 2020 •
1, 1,2
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

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Qui descriviamo un'immunocattura indiretta del panino ELISA per determinare il contenuto di glicoproteina immunogenica nei vaccini sulla rabbia. Questo test utilizza un anticorpo Monoclonal neutralizzante (mAb-D1) riconoscendo i trimer di lecoproteine. È un'alternativa al test NIH in vivo per seguire la coerenza della potenza del vaccino durante la produzione.

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Immunologia e Infezione Problema 159 Potenza del vaccino della rabbia test NIH metodo ELISA anticorpo monoclonale mAb-D1 trimers di glicoproteina contenuto di glicoproteina
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