JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
飞秒激光的光刺激激活目标细胞中的细胞外信号调节激酶(ERK)信号或线粒体事件
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells
Please note that all translations are automatically generated.
Click here for the English version.
飞秒激光的光刺激激活目标细胞中的细胞外信号调节激酶(ERK)信号或线粒体事件
DOI:
10.3791/59661-v
•
11:00 min
•
July 06, 2019
•
Shaoyang Wang
2
,
Minglie Hu
,
Tao Ren
,
Hao He
1
Department of Respiratory Medicine, Shanghai Sixth People’s Hospital, School of Medicine
,
Shanghai Jiao Tong University
,
2
Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering
,
Tianjin University
,
3
School of Biomedical Engineering
,
Shanghai Jiao Tong University
Chapters
00:04
Title
01:04
Setting Up the Photostimulation System
01:48
Preparing for Activation of ERK2 by Photostimulation
02:47
Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-A Mode
04:24
Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-B Mode
05:53
Activation of Mitoflashes and Other Mitochondrial Events by Photostimulation
06:55
Delivering Femtosecond Laser Stimulation into the Target Mitochondrion Using Stim-A Mode
08:32
Results: Activation of ERK Signaling or Mitochondrial Events in Target Cells
10:26
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
紧密聚焦的飞秒激光可以结合到共聚焦显微镜中,从而对细胞提供精确的刺激,从而实现实时观察和光刺激。光刺激可以激活细胞分子事件,包括ERK信号通路和反应氧物种的线粒体闪光。
Tags
Femtosecond Laser
Optical Manipulation
Cellular Signaling
ERK
Mitochondrial Events
Confocal Microscopy
Single-cell
Subcellular
Fluorescence Microscopy
Hela Cells
Transfection
Real-time Monitoring
Photo-bleaching
Photo-damage
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
成像摹蛋白偶联受体(GPCR)介导的信号,控制趋化
粘菌</em
脂筏的测定分区在活细胞中的荧光标记探针的荧光相关光谱(FCS)
FRET显微镜在活细胞单分子生物传感器的信号事件的实时监测
评估肾细胞蛋白激酶C同工酶,线粒体功能及细胞活力的
单线粒体超氧闪烁在完整心脏或共聚焦成像<em>在体内</em
实验方法来研究线粒体定位和核细胞周期激酶CDK1功能
成像G蛋白偶联受体介导的趋化和信令活动在中性粒细胞样细胞HL60
的细胞内信号与相邻小区发生细胞凋亡通过互动活细胞诱导活动标识
线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在
线粒体源肽治疗中 ERK 活化的亚细胞分离
Read Article