Journal
/
/
飞秒激光的光刺激激活目标细胞中的细胞外信号调节激酶(ERK)信号或线粒体事件
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells
DOI:

11:00 min

July 06, 2019

, , ,

Chapters

  • 00:04Title
  • 01:04Setting Up the Photostimulation System
  • 01:48Preparing for Activation of ERK2 by Photostimulation
  • 02:47Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-A Mode
  • 04:24Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-B Mode
  • 05:53Activation of Mitoflashes and Other Mitochondrial Events by Photostimulation
  • 06:55Delivering Femtosecond Laser Stimulation into the Target Mitochondrion Using Stim-A Mode
  • 08:32Results: Activation of ERK Signaling or Mitochondrial Events in Target Cells
  • 10:26Conclusion

Summary

Automatic Translation

紧密聚焦的飞秒激光可以结合到共聚焦显微镜中,从而对细胞提供精确的刺激,从而实现实时观察和光刺激。光刺激可以激活细胞分子事件,包括ERK信号通路和反应氧物种的线粒体闪光。

Related Videos

Read Article