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Voorbereiding van de muis netvlies Cryo-profielen voor Immunohistochemistry
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Voorbereiding van de muis netvlies Cryo-profielen voor Immunohistochemistry

Article DOI: 10.3791/59683-v • 05:25 min • July 1st, 2019
July 1st, 2019 •
1, 2, 2, 1
1Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, University of Pennsylvania School of Veterinary Medicine

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Dit rapport beschrijft uitgebreide methoden voor de voorbereiding van bevroren muis Retina secties voor Immunohistochemistry (IHC). Beschreven methoden omvatten dissectie van de oculaire posterior Cup, Paraformaldehyde fixatie, inbedding in optimale snij-temperatuur (OCT) media en weefsel oriëntatie, sectioneren en immunokleuring.

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Neurowetenschappen Retina Cryo-sectie optimale snij temperatuur media okt Immunohistochemistry Mouse Eye enucleation
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