Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

With the use of this protocol researchers can identify novel exome or extracellular vesicle based and tissue based micro cellular for advanced prostate cancer. Isolating RNA from both F-F-B tissues and extracellular vesicles is often challenging as most of it is either de created or is in limited quantity This particle will elaborate on different methods to optimize the R-N-A inputs and C-D-N-A libraries to gene most specific free and high quality data for next generation sequencing. Next gen sequencing offers the most comprehensive platform for understanding molecule alteration in tumor that underlie tumor progression this particle is optimized with prostate cancer clinical sample, and can provide novel insights into the underlying biology of advanced aggressive prostate cancer.

Optimizing the C-D-N-A libraries from low input material is a crucial step for a successful sequencing run it is therefore very important to generate high quality C-D-N-A libraries to get accurate results. After performing micro dissection of tissues as previously done proceed to isolating serum derived E-V's Thaw rows of patient serum samples at four degrees celsius. Transfer 110 micro liters of thawed serum sample to a tube and spin at two thousand times G for thirty minutes.