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JoVE Journal Bioengineering
Kontrollert stamme av 3D-hydrogeler under levende mikroskopiavbildning
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Kontrollert stamme av 3D-hydrogeler under levende mikroskopiavbildning

Article DOI: 10.3791/61671-v • 07:41 min • December 4th, 2020
December 4th, 2020 •
1, 2, 3, 3, 2, 3,4
1Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering, Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems, Tel-Aviv University

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Den presenterte metoden innebærer uniaxial strekking av 3D myke hydrogeler innebygd i silikongummi samtidig som levende konfokal mikroskopi tillates. Karakterisering av ytre og indre hydrogelstammer samt fiberjustering er demonstrert. Enheten og protokollen som er utviklet, kan vurdere cellenes respons på ulike strekkregimer.

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Bioingeniør Utgave 166 Hydrogel Ekstracellulær matrise Mekanobiologi Cellemekanikk Mekanisk kraft Ekstern strekking Fibrøst nettverk Fiberjustering
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