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Journal / Cancer Research / 人类乳腺上皮细胞致癌转化的体外评价…
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人类乳腺上皮细胞致癌转化的体外评价
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人类乳腺上皮细胞致癌转化的体外评价

Article DOI: 10.3791/61716-v • 09:44 min • September 24th, 2020
September 24th, 2020 •
1,2, 1,3,4, 5,6,7, 1, 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

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该协议提供实验体外工具，以评估人类乳腺细胞的转化。介绍了后续细胞增殖率、锚地独立生长能力以及3D培养基中细胞系系与地下室膜基质分布的详细步骤。

Tags

癌症研究， 问题 163， 细胞转化， 生长曲线， 锚定测定， 3D 细胞培养， 人类乳腺上皮细胞， 肿瘤发生
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