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Journal
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Cancer Research
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人类乳腺上皮细胞致癌转化的体外评价
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
人类乳腺上皮细胞致癌转化的体外评价
DOI:
10.3791/61716-v
•
09:44 min
•
September 24, 2020
•
Joan Repullés
2
,
Mariona Terradas
3,4
,
Gemma Fuster
6,7
,
Anna Genescà
,
Teresa Anglada
1
Department of Cell Biology, Physiology and Immunology
,
Universitat Autònoma de Barcelona
,
2
Optical Microscopy Core Facilities
,
IDIBELL
,
3
Hereditary Cancer Program, Catalan Institute of Oncology
,
IDIBELL
,
4
Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell)
,
IDIBELL
,
5
New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine
,
University of Barcelona (IBUB)
,
6
Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences
,
University of Barcelona
,
7
Department of Biosciences, Faculty of Sciences and Technology
,
University of Vic
Chapters
00:04
Introduction
00:42
Breast Primary Epithelial Cell Passaging and Population Doubling Calculation
02:12
Three-Dimensional Cell Culture in Basement Membrane Matrix and Immunofluorescent Protein Detection
05:07
Anchorage-Independent Assay and Colony Quantification
07:19
Results: Representative BPEC Transformation Evaluation
09:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议提供实验体外工具,以评估人类乳腺细胞的转化。介绍了后续细胞增殖率、锚地独立生长能力以及3D培养基中细胞系系与地下室膜基质分布的详细步骤。
Tags
In Vitro
Oncogenic Transformation
Human Mammary Epithelial Cells
Cell Culture
Cell Proliferation
3D Cell Culture
Acini-like Structures
Basement Membrane Matrix
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