Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

Rebecca E. Nakles; Sarah L. Millman; M. Carla Cabrera; Peter Johnson; Susette Mueller; Philipp S. Hoppe; Timm Schroeder; Priscilla A. Furth

doi:10.3791/50198

JoVE Journal
Medicine

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JoVE Journal Medicine

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

延时成像的主要癌前乳腺上皮细胞来源于遗传工程小鼠乳腺癌模型

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11:47 min

February 8, 2013

DOI: 10.3791/50198-v

Rebecca E. Nakles¹, Sarah L. Millman¹, M. Carla Cabrera¹, Peter Johnson^1,2, Susette Mueller^1,2, Philipp S. Hoppe³, Timm Schroeder³, Priscilla A. Furth^1,2,4,5

¹Department of Oncology,Georgetown University, ²Lombardi Comprehensive Cancer Center,Georgetown University, ³Stem Cell Dynamics,Helmholtz Zentrum München - German Research Center for Environmental Health, ⁴Department of Medicine,Georgetown University, ⁵Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science,Dankook University

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时间延时成像是用来评估初级癌前来自乳腺癌风险遗传工程小鼠模型，以确定是否有特定的行为参数之间的相关性和不同的遗传病变的乳腺上皮细胞的行为。

以下实验的总体目标是确定易患乳腺癌的遗传病变是否会改变原代培养中松果体塑料记忆细胞的行为。这是通过从基因工程小鼠中分离原代乳腺上皮细胞来实现的。作为第二步，在 5 到 7 天内进行活细胞延时成像，制作视频来分析细胞行为。

然后，使用跟踪单细胞的软件程序来量化和比较从不同基因工程乳腺癌小鼠模型中分离的细胞的行为。结果表明，特定的遗传病变在肿瘤前记忆中诱导不同的行为。基于使用单细胞追踪进行的定量行为分析的上皮细胞。

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