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Journal / Biochemistry / Spot Variation Fluorescence Correlation…
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Biochemistry

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JoVE Journal Biochemistry
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Article DOI: 10.3791/61823-v • 05:56 min • November 12th, 2020
November 12th, 2020 •
*1, *2, *1, 3, 4, 2, 1, 2
1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ
* These authors contributed equally

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Summary
Automatic Translation

This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

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Spot Variation Fluorescence Correlation Spectroscopy Plasma Membrane Living Cells Lateral Organization Innovative Techniques Time Resolution Spatial Resolution Non-invasive Physiological Conditions Iris Diaphragms Laser Power Alignment Detection Path Rhodamine 6G Solution Correlator Software Noise Level Waste Size Count Rate Molecule Per Second Spot Size Calibration
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