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1Division of Cardiology,EVK Düsseldorf, cNEP, cardiac Neuro- and Electrophysiology Research Consortium, 2DZHK (German Centre for Cardiovascular Research), 3Institute of Neural and Sensory Physiology, Medical Faculty,Heinrich Heine University Düsseldorf, 4Clinic for Cardiology, University Heart & Vascular Centre,University Hospital Hamburg-Eppendorf, 5Department of Cardiology,Leiden University Medical Center
Chapters
- 00:04Introduction
- 01:06Location and Dissection of Murine Stellate Ganglia
- 02:01Whole Mount Immunohistochemistry Protocol
- 05:23Imaging and Analyses of Murine Stellate Ganglia
- 05:44Results: Qualitative and Quantitative Analysis of Murine Stellate Ganglia
- 07:14Conclusion
Alterações fisiopatológicas no sistema nervoso autônomo cardíaco, especialmente em seu ramo simpático, contribuem para o surgimento e manutenção de arritmias ventriculares. No presente protocolo, mostramos como caracterizar gânglios estelares murinos para melhorar a compreensão dos processos moleculares e celulares subjacentes.
