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Journal / Genetics / 使用GAL4-UAS系统在冈比亚按蚊的功能遗传学中…
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Genetics

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JoVE Journal Genetics
使用GAL4-UAS系统在冈比亚按蚊的功能遗传学中
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使用GAL4-UAS系统在冈比亚按蚊的功能遗传学中

Article DOI: 10.3791/62131-v • 06:29 min • April 15th, 2021
April 15th, 2021 •
1, 1, 1,2,3, 1, 1, 1, 1
1Department of Vector Biology, Liverpool School of Tropical Medicine, 2IMBB FORTH, 3Department of Biology, University of Crete

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二分GAL4-UAS系统是一种多功能工具，用于以受控的时空方式修饰基因表达，从而允许在 冈比亚按蚊中进行功能性遗传分析。使用该系统所描述的程序是半标准化的克隆策略，性别化和蛹荧光蛋白标志物的筛选以及胚胎固定。

Tags

遗传学，第170期，3xP3，定位，内源性表达，RNAi敲低，显微镜检查，二分，杀虫剂耐药性，载体能力。
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