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Utilizzo dello strumento Minigene E1A per studiare le modifiche allo splicing dell'mRNA
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Utilizzo dello strumento Minigene E1A per studiare le modifiche allo splicing dell'mRNA

Article DOI: 10.3791/62181-v • 10:25 min • April 22nd, 2021
April 22nd, 2021 •
1, 1, 1
1Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas

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Questo protocollo presenta uno strumento rapido e utile per valutare il ruolo di una proteina con funzione insolita nella regolazione dello splicing alternativo dopo il trattamento chemioterapico.

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Cancer Research mRNA alternative splicing minigene E1A Nek4 chemioterapia response isoforms expression HEK293 stable cells.
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