01:02Ex Vivo T Cell Isolation from Peripheral Blood Mononuclear Cells (PBMCs)
02:47T Cell Stimulation and T Cell Expansion
03:54Transduction of Lentiviruses on Stimulated T Cells
05:10Surface Staining of B Cell Maturation Antigen (BCMA)-CAR Using Goat Anti-Mouse IgG (H+L) and Intracellular Staining of NIS Using Anti-Human ETNL NIS
07:18NIS+BCMA-CAR T Cell In Vivo Imaging Using BCMA+OPM-2 Xenograft Mouse Model
07:57Results: T Cell Growth Kinetics and In Vivo Cell Trafficking Assay
Detta protokoll beskriver metoden för icke-invasiv spårning T-celler genetiskt konstruerade för att uttrycka chimeric antigen receptorer in vivo med en kliniskt tillgänglig plattform.