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Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy
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Cancer Research
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JoVE Journal Cancer Research
Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

DOI:
10.3791/59949-v

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06:51 min

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December 17, 2019

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,
1Center for Cellular Immunotherapies,Perelman School of Medicine at the University of Pennsylvania

Chapters

  • 00:05Title
  • 01:09T Cell Activation, Transduction, and Expansion
  • 04:22Analysis of Manufactured Chimeric Antigen Receptor T cells
  • 05:50Conclusion

Summary

Automatic Translation

Automatic Translation

We describe an approach to reliably generate chimeric antigen receptor (CAR) T cells and test their differentiation and function in vitro and in vivo.

Tags

Keywords: CAR T CellsAdoptive ImmunotherapyT Cell ActivationLentiviral TransductionCryopreservation

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