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Biochemistry
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活植物细胞中的Förster共振能量转移测量
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Biochemistry
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JoVE Journal
Biochemistry
Förster Resonance Energy Transfer Measurements in Living Plant Cells
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活植物细胞中的Förster共振能量转移测量
DOI:
10.3791/62758-v
•
06:53 min
•
June 28, 2021
•
Sonja Michèle Schmidtpott
,
Thorsten Seidel
1
Dynamic Cell Imaging, Biochemistry and Physiology of Plants, Faculty of Biology
,
Bielefeld University
Chapters
00:04
Introduction
00:25
Laser Adjustment
02:03
Adjustment of Photomultipliers
02:57
Frster Resonance Energy Transfer (FRET) Image Acquisition
04:09
Determination of Crosstalk Corrections, Calibration of the Measurements, and Data Evaluation
05:13
Results: FRET Measurements in Living Plant Cells
06:22
Conclusion
Summary
Automatic Translation
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提供了一个方案,用于设置标准的共聚焦激光扫描显微镜,用于
体内
Förster共振能量转移测量,然后进行数据评估。
Tags
FRET
Förster Resonance Energy Transfer
Protoplasts
Laser Adjustment
Photomultiplier
Reflection Filter
Line Scanning
Histogram View
Detector Gain
Laser Intensity
Data Evaluation
Image Acquisition
Water Immersion Objective
Live Cells
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