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August 09, 2021
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This protocol describes an efficient method for dissociating sputum into a single cell suspension and the subsequent characterization of cellular subsets on standard flow cytometric platforms.
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Cite this Article
Grayson, M., Lai, S., Bederka, L. H., Araujo, P., Sanchez, J., Reveles, X. T., Rebel, V. I., Rebeles, J. Quality-Controlled Sputum Analysis by Flow Cytometry. J. Vis. Exp. (174), e62785, doi:10.3791/62785 (2021).
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