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Quality-Controlled Sputum Analysis by Flow Cytometry
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Quality-Controlled Sputum Analysis by Flow Cytometry

Quality-Controlled Sputum Analysis by Flow Cytometry

DOI:
10.3791/62785-v

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07:22 min

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August 09, 2021

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, , , , , , ,
1bioAffinity Technologies, 2Department of Cell Systems & Anatomy,The University of Texas Health Science Center at San Antonio

Chapters

  • 00:04Introduction
  • 01:00Sputum Dissociation
  • 02:30Antibody and Viability Dye Staining
  • 03:37Fixation with 1% Paraformaldehyde
  • 04:40Flow Cytometer – Data Acquisition
  • 05:33Results: Sputum Analysis by Flow Cytometry
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes an efficient method for dissociating sputum into a single cell suspension and the subsequent characterization of cellular subsets on standard flow cytometric platforms.

Tags

Keywords: Sputum AnalysisFlow CytometryQuality ControlCell CountCOPDAsthmaLung CancerCOVID-19NACDTTHBSSTrypan BlueHemocytometerCompensation TubesAntibody StainingDye Staining

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