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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
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Journal (Immunology and Infection)
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Journal (Immunology and Infection)
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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Article DOI: 10.3791/62985-v • 09:57 min • November 16th, 2021
November 16th, 2021 •
1,2, 1,2,3, 1,2, 1,2, 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

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Summary
Automatic Translation

In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.

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Flow Cytometric Analysis Identification Innate Immune Cells Adaptive Immune Cells Murine Lung Gating Strategies Comparison Of Results Comprehensive And Reproducible Gating Strategy Pulmonary Myeloid Immune Populations Non-myeloid Immune Populations Nine Markers Characterization Steady-state Conditions Disease Models Lung Immune Landscape Digestion Conditions Reagents Release Of Immune Cells Euthanized Animal Preparation Surgery Preparation Skin Removal Lung Flushing
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