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Immunology and Infection
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Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

DOI:
10.3791/57287-v

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09:09 min

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April 20, 2018

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, , ,
1Norton Thoracic Institute,St. Joseph’s Hospital and Medical Center, 2Flow Cytometry Core,St. Joseph’s Hospital and Medical Center

Chapters

  • 00:04Title
  • 00:50Isolation of Alveolar Macrophages (AMs) from Murine Bronchoalveolar Lavage (BAL) Fluid
  • 03:58Isolation of AMs from Mouse Lung: Single-cell Suspension
  • 06:04In Vitro Culture of AMs
  • 07:06Results: Characteristics of Mouse Alveolar Macrophages
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

This communication describes methodologies for isolation and culture of alveolar macrophages from humans and murine models for experimental purposes.

Tags

Keywords: Alveolar MacrophagesIn Vitro CultureMurineHumanLungBronchoalveolar LavagePulmonary ImmunologyRespiratory InfectionInflammationAutoimmunityCell IsolationTracheal CatheterizationPerfusion

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