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JoVE Journal Biochemistry
Förster Resonans Energy Transfer Mapping
A Polyaniline-based Sensor of Nucleic Acids
Journal (Biochemistry)
A Polyaniline-based Sensor of Nucleic Acids
Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
Journal (Biochemistry)
Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
 
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Förster Resonans Energy Transfer Mapping: En ny metode til at belyse globale strukturelle træk

Article DOI: 10.3791/63433-v • 07:09 min • March 16th, 2022
March 16th, 2022 •
1, 1,2, 1,2
1Molecular Biology and Biochemistry Department, Wesleyan University, 2Molecular Biophysics Program, Wesleyan University

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Undersøgelsen beskriver metoden til FRET-kortlægning, herunder valg af mærkningssteder, valg af farvestoffer, erhvervelse og dataanalyse. Denne metode er effektiv til bestemmelse af bindingssteder, konformationsændringer og dynamiske bevægelser i proteinsystemer og er mest nyttig, hvis den udføres sammen med eksisterende 3D-strukturelle oplysninger.

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Biokemi udgave 181 fluorescens energioverførsel SecA proteintranslokation FRET-kortlægning
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