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Isolamento das células epitélio pigmentadas do rato primário
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Isolamento das células epitélio pigmentadas do rato primário

Article DOI: 10.3791/63543-v • 06:21 min • November 4th, 2022
November 4th, 2022 •
1,2, 3, 1,2, 1,2,3
1Eye Research Institute, Oakland University, 2Eye Research Center (OUWB)/ERC, William Beaumont School of Medicine, 3Oral Biology and Diagnostic Sciences, Dental College of Georgia, Augusta University

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Este manuscrito descreve um protocolo simplificado para o isolamento de células de epitélio pigmentado da retina (RPE) a partir de olhos de rato de forma stepwise. O protocolo inclui a enucleação e dissecação dos olhos do rato, seguido pelo isolamento, semeadura e cultivo de células RPE.

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Biologia Edição 189
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