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Journal / Biochemistry / Zuivering van ubiquitinated p53-eiwitten uit…
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JoVE Journal Biochemistry
Zuivering van ubiquitinated p53-eiwitten uit zoogdiercellen
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Zuivering van ubiquitinated p53-eiwitten uit zoogdiercellen

Article DOI: 10.3791/63602-v • 10:55 min • March 21st, 2022
March 21st, 2022 •
1, 1, 1
1Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

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Het protocol beschrijft een stapsgewijze methode om ubiquitinated eiwitten uit zoogdiercellen te zuiveren met behulp van het p53 tumor suppressor-eiwit als voorbeeld. Ubiquitinated p53-eiwitten werden gezuiverd uit cellen onder strenge niet-condenserende en denaturerende omstandigheden.

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Biochemie nummer 181
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