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Biochemistry

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JoVE Journal Biochemistry
Purificación de proteínas p53 ubiquitinadas a partir de células de mamíferos
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Purificación de proteínas p53 ubiquitinadas a partir de células de mamíferos

Article DOI: 10.3791/63602-v • 10:55 min • March 21st, 2022
March 21st, 2022 •
1, 1, 1
1Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

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El protocolo describe un método paso a paso para purificar proteínas ubiquitinadas de células de mamíferos utilizando la proteína supresora de tumores p53 como ejemplo. Las proteínas p53 ubiquitinadas se purificaron de células bajo estrictas condiciones no desnaturalizantes y desnaturalizantes.

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Bioquímica Número 181
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