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原代细胞培养物研究小鼠穆勒胶质细胞在微RNA处理后的再生潜力
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment
DOI:

10:16 min

March 28, 2022

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Chapters

  • 00:05Introduction
  • 00:48Eyeball Cleaning, Retina Extraction, Transfer, and Washing
  • 03:06Retina Dissociation
  • 05:20Cell Passage to Remove Neuronal Survivors
  • 07:12Results: Tracking of Mller Glia Conversion into Progenitor Cells and Neuronal Identity Validation
  • 09:24Conclusion

Summary

Automatic Translation

从小鼠视网膜获得的Müller神经胶质细胞原代培养物是研究microRNA处理后神经胶质转化为视网膜祖细胞的非常强大和可靠的工具。在随后应用 体内 方法之前,可以测试单个分子或组合。

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