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Developmental Biology
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原代细胞培养物研究小鼠穆勒胶质细胞在微RNA处理后的再生潜力
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JoVE Journal
Developmental Biology
Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment
Please note that all translations are automatically generated.
Click here for the English version.
原代细胞培养物研究小鼠穆勒胶质细胞在微RNA处理后的再生潜力
DOI:
10.3791/63651-v
•
10:16 min
•
March 28, 2022
•
Seoyoung Kang
,
Stefanie G. Wohl
1
Department of Biological and Vision Sciences, College of Optometry
,
The State University of New York
Chapters
00:05
Introduction
00:48
Eyeball Cleaning, Retina Extraction, Transfer, and Washing
03:06
Retina Dissociation
05:20
Cell Passage to Remove Neuronal Survivors
07:12
Results: Tracking of Mller Glia Conversion into Progenitor Cells and Neuronal Identity Validation
09:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
从小鼠视网膜获得的Müller神经胶质细胞原代培养物是研究microRNA处理后神经胶质转化为视网膜祖细胞的非常强大和可靠的工具。在随后应用
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方法之前,可以测试单个分子或组合。
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