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Coleta remota de dados de tomografia crio-elétron e média de subtomograma
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Coleta remota de dados de tomografia crio-elétron e média de subtomograma

Article DOI: 10.3791/63923-v • 08:55 min • July 12th, 2022
July 12th, 2022 •
1, 1, *1, *1,2,3
1Electron Bio-Imaging Centre, Diamond Light Source Ltd, Harwell Science & Innovation Campus, 2Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, 3Chinese Academy of Medical Sciences Oxford Institute, University of Oxford
* These authors contributed equally

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O presente protocolo descreve a aquisição remota de dados remotos crio-elétrons de alta resolução usando Tomo5 e processamento de dados subsequente e média de subtomogramas usando a emClarity. A apoferritina é usada como exemplo para ilustrar processos passo a passo detalhados para alcançar uma estrutura crio-ET com resolução de 2,86 Å.

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Biologia Edição 185
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