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Testen der Epithelpermeabilität in fetalen Gewebe-abgeleiteten Enteroiden
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Testen der Epithelpermeabilität in fetalen Gewebe-abgeleiteten Enteroiden

Article DOI: 10.3791/64108-v • 07:51 min • June 16th, 2022
June 16th, 2022 •
1, 2, 3, 3, 2
1Morsani College of Medicine, University of South Florida, 2Department of Pediatrics, Morsani College of Medicine, University of South Florida, 3Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida

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Dieses Protokoll beschreibt die Etablierung von Enteroiden, einem dreidimensionalen Darmmodell, aus fetalem Darmgewebe. Immunfluoreszierende Bildgebung von epithelialen Biomarkern wurde zur Modellcharakterisierung verwendet. Die apikale Exposition von Lipopolysacchariden, einem bakteriellen Endotoxin, induzierte die epitheliale Permeabilität in einer dosisabhängigen Weise, gemessen durch das Austreten von fluoreszierendem Dextran.

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Medizin Ausgabe 184
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