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Journal / Immunology and Infection / Aislamiento y cuantificación del virus de…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Aislamiento y cuantificación del virus de Epstein-Barr de la línea celular P3HR1
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Aislamiento y cuantificación del virus de Epstein-Barr de la línea celular P3HR1

Article DOI: 10.3791/64279-v • 09:14 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1,2
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut

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Este protocolo permite el aislamiento de partículas del virus de Epstein-Barr de la línea celular humana P3HR1 al inducir el ciclo lítico viral con forbol 12-miristato 13-acetato. Posteriormente, el ADN se extrae de la preparación viral y se somete a PCR en tiempo real para cuantificar la concentración de partículas virales.

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Inmunología e infección Número 187
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