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Journal / Immunology and Infection / Isolering och kvantifiering av Epstein-Barr-virus …
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolering och kvantifiering av Epstein-Barr-virus från P3HR1-cellinjen
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Isolering och kvantifiering av Epstein-Barr-virus från P3HR1-cellinjen

Article DOI: 10.3791/64279-v • 09:14 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1,2
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut

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Detta protokoll tillåter isolering av Epstein-Barr-viruspartiklar från den mänskliga P3HR1-cellinjen vid inducering av den virala lytiska cykeln med phorbol 12-myristate 13-acetat. DNA extraheras därefter från viruspreparatet och utsätts för PCR i realtid för att kvantifiera den virala partikelkoncentrationen.

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Immunologi och infektion utgåva 187
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