Abstract
An erratum was issued for: Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. The Protocol was updated.
Step 6.1.4.2 of the Protocol was updated from:
If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD600 to CFU conversion determined previously20) using this equation:
E = ((O-B) x V x D)/C
where E = volume to resuspend pellet in (μl), O = OD600 bacteria, B = OD600 blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD600 of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B".
Note: The predetermined constants (units OD600, normalized to 107 CFU ml-1, constants derived in20) are as follows: A. tropicalis (0.052), A. pomorum (0.038), L. brevis (0.056), L. plantarum (0.077).
to:
If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD600 to CFU conversion determined previously20) using this equation:
E = ((O-B) x V x D)/C
where E = volume to resuspend pellet in (μl), O = OD600 bacteria, B = OD600 blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD600 of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B".
Note: The predetermined constants (units OD600, normalized to 107 CFU ml-1, constants derived in20) are as follows: A. tropicalis (0.053), A. pomorum (0.038), L. brevis (0.077), L. plantarum (0.056).
Protocol
An erratum was issued for: Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. The Protocol was updated.
Step 6.1.4.2 of the Protocol was updated from:
If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD600 to CFU conversion determined previously20) using this equation:
E = ((O-B) x V x D)/C
where E = volume to resuspend pellet in (μl), O = OD600 bacteria, B = OD600 blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD600 of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B".
Note: The predetermined constants (units OD600, normalized to 107 CFU ml-1, constants derived in20) are as follows: A. tropicalis (0.052), A. pomorum (0.038), L. brevis (0.056), L. plantarum (0.077).
to:
If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD600 to CFU conversion determined previously20) using this equation:
E = ((O-B) x V x D)/C
where E = volume to resuspend pellet in (μl), O = OD600 bacteria, B = OD600 blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD600 of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B".
Note: The predetermined constants (units OD600, normalized to 107 CFU ml-1, constants derived in20) are as follows: A. tropicalis (0.053), A. pomorum (0.038), L. brevis (0.077), L. plantarum (0.056).
Disclosures
No conflicts of interest declared.
