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Journal / Biochemistry / Detection and Quantification of Calcitonin…
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JoVE Journal Biochemistry
Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
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Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Article DOI: 10.3791/64775-v • 07:14 min • June 16th, 2023
June 16th, 2023 •
1,2, 1,3, 1, 4, 5, 1, 6, 1,3, 1
1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine

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Summary
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Published data pertaining to calcitonin gene-related peptide (CGRP) concentrations in human plasma are inconsistent. These inconsistencies may be due to the lack of a standardized, validated methodology to quantify this neuropeptide. Here, we describe a validated enzyme-linked immunosorbent assay (ELISA) protocol to purify and quantify CGRP in human plasma.

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Detection Quantification Calcitonin Gene-related Peptide (CGRP) Human Plasma Modified Enzyme-linked Immunosorbent Assay (ELISA) Purification Quantification Methodologies Sample Processing Protease Degradation Solid Phase Extraction Non-specific Binding Blocking Buffer Microtiter Plates Migraine Biomarker Status Cardiologic Diseases Dermatologic Diseases Viral Diseases COVID-19 Whole Blood Collection Antecubital Vein Venipuncture Methods Aprotinin Competitive Serine Protease Inhibitor Centrifugation Plasma Fraction Cryo Vial Extraction Cartridge
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