Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

Christina M. Kaszuba; Benjamin J. Rodems; Sonali Sharma; Edgardo I. Franco; John M. Ashton; Laura M. Calvi; Jeevisha Bajaj

doi:10.3791/66093

Journal

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Cancer Research

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Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

1,063 Views

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06:33 min

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November 10, 2023

DOI:

10.3791/66093-v

DOI:

10.3791/66093-v

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06:33 min

November 10, 2023

•

1 Views

Christina M. Kaszuba^1,2, Benjamin J. Rodems^1,3, Sonali Sharma^1,3, Edgardo I. Franco^1,2, John M. Ashton^1,3,4, Laura M. Calvi^1,5, Jeevisha Bajaj^1,3

¹Wilmot Cancer Institute,University of Rochester Medical Center, ²Department of Biomedical Engineering,University of Rochester, ³Department of Biomedical Genetics,University of Rochester Medical Center, ⁴Genomics Research Center,University of Rochester Medical Center, ⁵Division of Endocrinology and Metabolism, Department of Medicine,University of Rochester Medical Center

Transcript

Our research aims to explore how the bone marrow’s changing environment influences tumor growth. We developed a flow cytometry protocol to analyze the non-immune cells in bone marrow and bone tissues, focusing on murine hematological malignancies. This method is adaptable for studying bone marrow in various murine models.

Our study focuses on isolating and identifying alterations in bone marrow stromal populations during cancer progression. Understanding their impact on disease onset and advancement could pave the way for new treatments that target these cancer-facilitating changes in the bone marrow stromal cells. Our protocol uniquely combines mechanical and enzymatic digestions, magnetic depletions, and flow cytometry to delineate the bone marrow microenvironment cellular makeup.

It stands out by employing magnetic separation and incorporating cells from both bone and marrow, offering a more comprehensive representation of this microenvironment.

Summary

Here a detailed protocol to isolate and characterize bone marrow microenvironmental populations from murine models of myelodysplastic syndromes and acute myeloid leukemia is presented. This technique identifies changes in the non-hematopoietic bone marrow niche, including the endothelial and mesenchymal stromal cells, with disease progression.