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Journal / Biology / Sustrato Generación para endonucleasas de CRISPR…
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Sustrato Generación para endonucleasas de CRISPR / Cas Sistemas
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Sustrato Generación para endonucleasas de CRISPR / Cas Sistemas

Article DOI: 10.3791/4277-v • 11:53 min • September 8th, 2012
September 8th, 2012 •
1, 1, 1, 1, 1
1Prokaryotic Small RNA Biology, Max-Planck-Institute for Terrestrial Microbiology

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CRISPR / Cas sistemas median la inmunidad adaptativa en bacterias y arqueas. Muchas proteínas CAS se propone actuar como endoribonucleases que actúan sobre los precursores crRNA de longitud variable. Aquí se ilustran tres enfoques diferentes para generar pre-crRNA sustratos para el análisis bioquímico de la actividad endonucleasa de Cas.

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La biología molecular número 67 CRISPR / Cas endonucleasa, crRNA CAS6
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