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Overflate Passivisering for single-molekyl Protein Studies
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Overflate Passivisering for single-molekyl Protein Studies

Article DOI: 10.3791/50549-v • 10:35 min • April 24th, 2014
April 24th, 2014 •
1, 1, 2, 2, 2, 1
1Kavli Institute of NanoScience, Department of BioNanoScience, Delft University of Technology, 2Department of Chemistry, Seoul National University

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Vi beskriver en fremgangsmåte for passivering en glassflate ved bruk av polyetylenglykol (PEG). Denne protokollen omfatter rengjøring overflate, overflaten funksjon, og PEG belegg. Vi introduserer en ny strategi for å behandle overflaten med PEG molekyler over to runder, som gir suveren kvalitet av passivisering i forhold til eksisterende metoder.

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Chemistry enkelt-molekyl-spektroskopi polymer polyetylen-glykol (PEG) piranha etsing amino-silanization overflatepassivering fluorescens glassoverflatebelegg.
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