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ライブの検出
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
Journal (Biology)
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
Isolation and Preparation of Endosomes Containing Magnetic Nanoparticles from Cultured Cells (Video) | JoVE
Journal (Biology)
Isolation and Preparation of Endosomes Containing Magnetic Nanoparticles from Cultured Cells (Video) | JoVE
 
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ライブの検出

Article DOI: 10.3791/50967-v • 08:16 min • February 1st, 2014
February 1st, 2014 •
1, 1, 1
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology, Food and Drug Administration

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定量PCRアッセイは、 大腸菌 O157を検出するために開発されました：H7は、固有の遺伝子マーカー、Z3276をターゲットに。定量PCRは、生細胞を検出するためのプロピジウムモノアジド（PMA）処理と合わせた。このプロトコルは、多数のサンプルの容易かつ一貫性のある操作のための96ウェルプレートフォーマットに変更され、適応されてい

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微生物学、84号、プロピジウムモノアジド（PMA）、リアルタイムPCR、
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