2D og 3D Kromosom Maleri i malariamygg

Immunology and Infection
 

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George, P., Sharma, A., Sharakhov, I. V. 2D and 3D Chromosome Painting in Malaria Mosquitoes. J. Vis. Exp. (83), e51173, doi:10.3791/51173 (2014).

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Abstract

Materials

Name Company Catalog Number Comments
Acetic acid Fisher Scientific A491-212
Methanol Fisher Scientific A412-4
Propionic acid  Sigma-Aldrich 402907
Membrane slides 1.0 PET Zeiss 415190-9051-000
Buffer tablets “GURR” Life Technologies 10582-013
KaryoMAX Giemsa Stain Life Technologies 10092-013
ThermoBrite Slide Denaturation/Hybridization System Abbott Molecular 30-144110
Vacufuge vacuum concentrator Eppendorf 22820001
Spectroline Microprocessor-Controlled UV Crosslinker XL-1000 Fisher Scientific 11-992-89
Thermo Scientific NanoDrop Fisher Scientific ND-2000
REPLI-g Single Cell Kit Qiagen 150343
GenomePlex Single Cell Kit (WGA4) Sigma-Aldrich WGA4-10RXN
GenomePlex WGA Reamplification Kit (WGA3) Sigma-Aldrich WGA3-50RXN
MZ6 Leica stereomicroscope Leica VA-OM-E194-354 A different stereomicroscope can be used
Olympus CX41 Phase Microscope Olympus CX41RF-5 A different phase microscope can be used
PALM MicroBeam Laser Microdissection Microscope Zeiss
Thermomixer Eppendorf 22670000
10x PBS Invitrogen P5493
50x Denhardt’s solution Sigma-Aldrich D2532
99% Formamide Fisher Scientific BP227500
Dextran sulfate sodium salt Sigma D8906
20x SSC buffer Invitrogen AM9765
ProLong Gold antifade reagent with DAPI Invitrogen P-36931
dATP, dCTP, dGTP, dTTP Fermentas R0141, R0151, R0161, R0171
Cy3-dUTP, Cy5-dUTP GE Healthcare PA53022, PA55022
BSA Sigma-Aldrich A3294
DNA polymerase I Fermentas EP0041
DNase I  Fermentas EN0521
Spermine Sigma-Aldrich S3256-1G
Spermidine Sigma-Aldrich S0266-1G
Potassium Chloride (KCl) Fisher Scientific BP366-500
Sodium Chloride (NaCl) Fisher Scientific BP3581
EGTA Sigma-Aldrich E0396-25G
PIPES Sigma-Aldrich P6757-25G
EDTA Fisher Scientific S311-500
Digitonin Sigma-Aldrich D141-100MG
Triton-X100 Fisher Scientific BP151-100
AdhesiveCap 500 clear Zeiss 415190-9211-000
QIAamp DNA Micro Kit (50) Qiagen 56304
Genomic DNA Clean and Concentrator Kit Zymo Research D4010
37% Paraformaldehyde Fisher Scientific F79-500

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References

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